HerbELICO® and scientific research

Nature and Science

HerbELICO®

herbal preparation is a result of a seven-year long scientific research, conducted by a team of scientists, medical doctors and university professors from Serbia, Hungary and Australia, focusing on how essential oils of aromatic plants can defeat Helicobacter pylori (H. pylori).

MORE ABOUT SUPPLEMENT

HerbELICO®

 herbal preparation is a result of a seven-year long scientific research, conducted by a team of scientists, medical doctors and university professors from Serbia, Hungary and Australia, focusing on how essential oils of aromatic plants can defeat Helicobacter pylori (H. pylori).

MORE ABOUT SUPPLEMENT

At the very beginning of the study, 14 aromatic plants (hyssop, sage, rosemary, fennel, yarrow, tarragon, basil, juniper, wild thyme, lemon balm, lavender, mint, oregano and savory), growing in the Balkan peninsula were chosen and their essential oils were isolated.

The essential oils obtained were tested against H. pylori cultured in the laboratory.

Results showed that savory and oregano essential oils expressed the highest activity, whilst a combined mixture exhibited 4 times higher activity than individual oils. [1,2]

Since the mixture of savory and oregano essential oils had a significantly higher activity in the elimination of H. pylori than individual oils, the mixture was examined in the next phase.

Heliko bakterija prirodni lek

At the very beginning of the study, 14 aromatic plants (hyssop, sage, rosemary, fennel, yarrow, tarragon, basil, juniper, wild thyme, lemon balm, lavender, mint, oregano and savory), growing in the Balkan peninsula were chosen and their essential oils were isolated.

The essential oils obtained were tested against H. pylori cultured in the laboratory.

Results showed that savory and oregano essential oils expressed the highest activity, whilst a combined mixture exhibited 4 times higher activity than individual oils. [1,2]

Since the mixture of savory and oregano essential oils had a significantly higher activity in the elimination of H. pylori than individual oils, the mixture was examined in the next phase.

Heliko bakterija prirodni lek
Heliko bakterija prirodni lek

Research on laboratory animals came next.

Laboratory mice were infected with H. pylori.

Upon developed infection, mice were treated by the mixture of savory and oregano essential oils for 14 days.

The treatment was successful in 70% of cases, where complete elimination of H. pylori was proven.

The same research showed that the combined mixture of savory and oregano essential oils had no side effects: it was not cytotoxic, did not cause any immunomodulatory or allergic effects, did not alter liver enzymes ALT and AST, and did not induce expression of cytochrome P450 and heme oxygenase 1. [3]

These results encouraged us to continue our research.

Research on laboratory animals came next.

Laboratory mice were infected with H. pylori.

Upon developed infection, mice were treated by the mixture of savory and oregano essential oils for 14 days.

The treatment was successful in 70% of cases, where complete elimination of H. pylori was proven.

The same research showed that the combined mixture of savory and oregano essential oils had no side effects: it was not cytotoxic, did not cause any immunomodulatory or allergic effects, did not alter liver enzymes ALT and AST, and did not induce expression of cytochrome P450 and heme oxygenase 1. [3]

These results encouraged us to continue our research.

Heliko bakterija prirodni lek

To increase the efficacy, thyme essential oil was added to the mixture of savory and oregano essential oils, since it has proven to have strong anti-H. pylori activity. [4]

Doing this enriched the mixture with additional compounds with antimicrobial activity and thus the possibility for the bacterium to develop resistance was reduced.

The mixture with a strictly defined chemical composition, was named HerbELICO® and was used in further research.

To increase the efficacy, thyme essential oil was added to the mixture of savory and oregano essential oils, since it has proven to have strong anti-H. pylori activity. [4]

Doing this enriched the mixture with additional compounds with antimicrobial activity and thus the possibility for the bacterium to develop resistance was reduced.

The mixture with a strictly defined chemical composition, was named HerbELICO® and was used in further research.

The effect of HerbELICO® formula on different strains of H. pylori, isolated from people from all around the world and from different continents (China, India, Europe, Middle East, Africa) was investigated.

The tested strains of H. pylori were different in terms of their resistance to antibiotics. Some of them were resistant to antibiotics commonly used in the treatment of H. pylori infection, while others were not.

HerbELICO® had the same power to eliminate both antibiotic-resistant and antibiotic-non-resistant strains of H. pylori!

Also, HerbELICO® had the same power to eliminate all strains of H. pylori originating from different continents! [5]

These results showed that HerbELICO® is equally suitable for use anywhere in the world!

Heliko bakterija prirodni lek

In the next experiment, the time needed for complete elimination of H. pylori cultured in the laboratory by HerbELICO® was measured.

It has been shown that HerbELICO® kills H. pylori almost immediately after they come into contact and no live bacteria is detected 10 minutes post-incubation.

An additional problem which causes difficulties in H. pylori treatment is that the inner wall of the stomach is lined by gastric mucin. H. pylori can hide from attackers (antibiotics/HerbELICO®) behind a layer of thick gastric mucin.

Drugs that cannot penetrate gastric mucin are unsuccessful in the treatment of H. pylori infection.

Therefore, it was necessary to examine whether HerbELICO® could pass through gastric mucin.

It has been proven that HerbELICO® successfully crosses gastric mucin and kills almost all bacteria on the other side of the mucin barrier after only 30 minutes. [5]

The effect of HerbELICO® formula on different strains of H. pylori, isolated from people from all around the world and from different continents (China, India, Europe, Middle East, Africa) was investigated.

The tested strains of H. pylori were different in terms of their resistance to antibiotics. Some of them were resistant to antibiotics commonly used in the treatment of H. pylori infection, while others were not.

HerbELICO® had the same power to eliminate both antibiotic-resistant and antibiotic-non-resistant strains of H. pylori!

Also, HerbELICO® had the same power to eliminate all strains of H. pylori originating from different continents! [5]

These results showed that HerbELICO® is equally suitable for use anywhere in the world!

Heliko bakterija prirodni lek

In the next experiment, the time needed for complete elimination of H. pylori cultured in the laboratory by HerbELICO® was measured.

It has been shown that HerbELICO® kills H. pylori almost immediately after they come into contact and no live bacteria is detected 10 minutes post-incubation.

An additional problem which causes difficulties in H. pylori treatment is that the inner wall of the stomach is lined by gastric mucin. H. pylori can hide from attackers (antibiotics/HerbELICO®) behind a layer of thick gastric mucin.

Drugs that cannot penetrate gastric mucin are unsuccessful in the treatment of H. pylori infection.

Therefore, it was necessary to examine whether HerbELICO® could pass through gastric mucin.

It has been proven that HerbELICO® successfully crosses gastric mucin and kills almost all bacteria on the other side of the mucin barrier after only 30 minutes. [5]

Based on all our obtained results, we concluded that HerbELICO® effectively kills H. pylori and successfully passes through gastric mucin without exhibiting any side effects. This was enough for us to further focus on the development of the suitable pharmaceutical form of HerbELICO®.

In cooperation with the pharmaceutical company, Phytonet Ltd. (https://phytonet.com/) we tested several pharmaceutical forms, chose the most suitable and registered HerbELICO® as a dietary supplement in Serbia.

prirodno-lecenje-Helikobakterije

Now our HerbELICO® dietary supplement was ready for a Customer satisfaction Survey – evaluation of HerbELICO® effectiveness to eliminate H. pylori in people with a confirmed infection.

The Survey included 50 users. All users had a confirmed, acute or moderate H. pylori infection.

Ten days after the therapy started, 80% of users stated that they did not have the symptoms of H. pylori infection any more, whilst the other 20% of users lost symptoms by the end of the therapy.

None of the users noticed any side effects during the therapy.

Amongst 40 users that received the entire therapy and were tested sixty days after the therapy ended, 32 had a negative stool test regarding the presence of H. pylori.

Based on these results, it was concluded that HerbELICO® formula is extremely effective in elimination of H. pylori from the human stomach, when administered alone in the prescribed dosage regimen.

The success rate of HerbELICO® is estimated at 80%.

Recommendation to other users whose stool test after therapy was positive for the presence of H. pylori, although they subjectively felt much better, was to continue treatment with combined therapy: HerbELICO® + antibiotics.

The Survey was done by the company HerbElixa Ltd. in cooperation with the Medical laboratory Polarislab (www.polarislab.rs) from Novi Sad, Serbia.

We submitted an international patent application in order to protect our IP regarding HerbELICO® composition, usage and dosage in humans (we are waiting for a decision).

In addition, we submitted an application for EU trademark protection regarding HerbELICO® product name (we are waiting for a decision).

Heliko bakterija prirodni lek

Reference

  1. Lesjak et al. (2016): Binary and tertiary mixtures of Satureja hortensis and Origanum vulgare essential oils as potent antimicrobial agents against Helicobacter pylori. Phytother Res 30:476-484.
  2. Knezevic et al. (2018): A colorimetric broth microdilution method for assessment of Helicobacter pylori sensitivity to antimicrobial agents. J Pharma Biomed Analysis, 152: 271-278.
  3. Harmati et al. (2017): Binary mixture of Satureja hortensis and Origanum vulgare subsp. hirtum essential oils: in vivo therapeutic efficiency against Helicobacter pylori infection. Helicobacter e12350.
  4. Dandlen et al. (2011): Antimicrobial activity, cytotoxicity and intracellular growth inhibition of Portuguese Thymus essential oils. Rev Bras Farmacogn, 21: 1012–1024.
  5. Results of research conducted on Marshall Centre for Infectious Diseases Research and Training, University of Western Australia.

Based on all our obtained results, we concluded that HerbELICO® effectively kills H. pylori and successfully passes through gastric mucin without exhibiting any side effects. This was enough for us to further focus on the development of the suitable pharmaceutical form of HerbELICO®.

In cooperation with the pharmaceutical company, Phytonet Ltd. (https://phytonet.com/) we tested several pharmaceutical forms, chose the most suitable and registered HerbELICO® as a dietary supplement in Serbia.

prirodno-lecenje-Helikobakterije

Now our HerbELICO® dietary supplement was ready for a Customer satisfaction Survey – evaluation of HerbELICO® effectiveness to eliminate H. pylori in people with a confirmed infection.

The Survey included 50 users. All users had a confirmed, acute or moderate H. pylori infection.

Ten days after the therapy started, 80% of users stated that they did not have the symptoms of H. pylori infection any more, whilst the other 20% of users lost symptoms by the end of the therapy.

None of the users noticed any side effects during the therapy.

Amongst 40 users that received the entire therapy and were tested sixty days after the therapy ended, 32 had a negative stool test regarding the presence of H. pylori.

Based on these results, it was concluded that HerbELICO® formula is extremely effective in elimination of H. pylori from the human stomach, when administered alone in the prescribed dosage regimen.

The success rate of HerbELICO® is estimated at 80%.

Recommendation to other users whose stool test after therapy was positive for the presence of H. pylori, although they subjectively felt much better, was to continue treatment with combined therapy: HerbELICO® + antibiotics.

The Survey was done by the company HerbElixa Ltd. in cooperation with the Medical laboratory Polarislab (www.polarislab.rs) from Novi Sad, Serbia.

We submitted an international patent application in order to protect our IP regarding HerbELICO® composition, usage and dosage in humans (we are waiting for a decision).

In addition, we submitted an application for EU trademark protection regarding HerbELICO® product name (we are waiting for a decision).

Heliko bakterija prirodni lek

Reference

  1. Lesjak et al. (2016): Binary and tertiary mixtures of Satureja hortensis and Origanum vulgare essential oils as potent antimicrobial agents against Helicobacter pylori. Phytother Res 30:476-484.
  2. Knezevic et al. (2018): A colorimetric broth microdilution method for assessment of Helicobacter pylori sensitivity to antimicrobial agents. J Pharma Biomed Analysis, 152: 271-278.
  3. Harmati et al. (2017): Binary mixture of Satureja hortensis and Origanum vulgare subsp. hirtum essential oils: in vivo therapeutic efficiency against Helicobacter pylori infection. Helicobacter e12350.
  4. Dandlen et al. (2011): Antimicrobial activity, cytotoxicity and intracellular growth inhibition of Portuguese Thymus essential oils. Rev Bras Farmacogn, 21: 1012–1024.
  5. Results of research conducted on Marshall Centre for Infectious Diseases Research and Training, University of Western Australia.